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. Author manuscript; available in PMC: 2012 Mar 25.
Published in final edited form as: Chem Biol. 2011 Mar 25;18(3):336–343. doi: 10.1016/j.chembiol.2010.12.009

Figure 4.

Figure 4

Relative activities of native hepcidin and the alanine mutants (A), His3 mutants (B), Phe4 mutants (C) and Ile6 mutants (D). HEK293T cells stably expressing ferroportin-GFP construct were treated with peptide (0.3 μM) for 24 h and the intensity of green fluorescence was then measured by FACS and expressed as a percentage relative to native hepcidin.