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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: J Biol Inorg Chem. 2010 Oct 31;16(2):285–297. doi: 10.1007/s00775-010-0725-z

Fig. 1.

Fig. 1

Colony-staining assay with the cytochrome caa3-specific substrate TMPD. a The B. subtilis strains: ORB6556, Sco::erm (Sco knockout mutant) (1); ORB6625, Sco::erm amyE::Sco (Sco knockout complemented with WT Sco gene) (2); ORB6893, Sco::erm amyE:: Sco[H135M] (Sco knockout complemented with H135M Sco gene) (3). b Western blot analysis of Sco expression in B. subtilis. Lane 1 JH642 (WT), lane 2 ORB6556 (sco knockout), lane 3 ORB6625 (sco knockout complemented with WT Sco gene), and lane 4 ORB6893 (Sco::erm amyE:: Sco[H135M])