Table 1.
Sample | KA |
---|---|
Wild type | 1.1 ± 0.8 × 1013 |
H135A | 8.0 ± 2.2 × 1010 |
H135M | 6.0 ± 3.5 × 1012 |
H135SeM | 2.1 ± 0.2 × 1012 |
I-H135M | 3.1 ± 1.2 × 1012 |
I-H135SeM | 2.8 ± 0.3 × 1012 |
Binding constants are the average of a number of titrations in both the forward and reverse directions, as listed in Table S1 of the ESM