Table 4.
Simulated CW X-EPR data for the Cu(II) species of the H135M variant in comparison with the WT and H135A Cu(II) species
| WT | H135A | H135M | ||
|---|---|---|---|---|
| gx | 2.0342(3) | 2.0324(2) | 2.0203(5) | 2.0300(5) |
| gy | 2.0288(3) | 2.0355 (2) | 2.0306(5) | 2.0260(5) |
| gz | 2.1503(3) | 2.1646(2) | 2.1142(5) | 2.1273(5) |
| Ax (Cu) | −135(2) | −145(2) | −130(3) | −166(5) |
| Ay (Cu) | −115(2) | −113(2) | −144(5) | −94(3) |
| Az (Cu) | −572(2) | −569(2) | −510(5) | −524(5) |
| Ax (14N) | 30 | |||
| Ay (14N) | 41 | |||
| Az (14N) | 31 | |||
| Ax (H) | 21 | 16 | 46 | 13 |
| Ay (H) | 16 | 14 | 19 | 16 |
| Az (H) | 18 | 11 | 56 | 26 |
| wx | 5.3 | 7.2 | 27 | 15 |
| wy | 6.9 | 5.5 | 15 | 9 |
| wz | 5.9 | 8.9 | 51 | 49 |
Hyperfine principal values in MHz (for units of 10−4 cm−1, divide by 3). Linewidths are in Gauss