Figure 2.
Comparison of the dose dependent increase of CYP24 mRNA by 1,25(OH)2D3 and 1,25(OH)2D3-3-BE. A498 cells were treated with ethanol control or 10−10-6 M of 1,25(OH)2D3 and 1,25(OH)2D3-3-BE for 24 hours, total RNA was prepared and CYP24 mRNA levels assessed by RT-PCR. β-Actin mRNA levels were determined for each sample as control. The results are representative of two independent experiments.
