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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Free Radic Biol Med. 2011 Mar 4;50(9):1163–1170. doi: 10.1016/j.freeradbiomed.2011.02.001

Figure 6.

Figure 6

EICs of m/z 266, m/z 296, m/z 324, and m/z 354 as POBN adducts of corresponding radicals detected in cellular peroxidation. Reaction conditions: radical adducts are generated from cells (~107 cells/mL, HCT-7 colony 29)/PBS with addition of 50 mM POBN and 1.0 mM DGLA after an incubation of about 30 min. The reaction in the cells was stopped by adding ACN (1:1, v/v) and supernatant was then condensed for LC/MS analysis. (A) EIC of m/z 266; (B) EIC of m/z 296; (C) EIC of m/z 324; and (D) EIC of m/z 354. Note that retention as well as MS spectra from cell peroxidation all agreed with the LC/MS analysis of COX-DGLA in in vitro experiments in Figures 2–5, and that m/z 266, m/z 296, m/z 324, and m/z 354 could be detected at relatively low EIC intensities when 50 mM POBN as well as 0.1, 0.25, and 0.5 mM DGLA were used in the cell-PBS incubation (cell viability of experiment of 1.0 mM to 0.1 mM DGLA was 90% to 10%, respectively).