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. Author manuscript; available in PMC: 2012 Mar 8.
Published in final edited form as: Cancer Cell. 2011 Mar 8;19(3):333–346. doi: 10.1016/j.ccr.2011.01.037

Figure 8. MMP-2-specific CD4+ T cell differentiation depends on IL-12 and OX40L expression by DCs.

Figure 8

(A) Cord blood-derived CD4+/CD25− cells from 19 donors were stimulated with autologous DCs previously loaded with active MMP-2 (MMP-2 enzymatic activity was controlled (Fig.S5C-D)) for 15 days in the absence or in the presence of exogenous rhIL-12 (10ng/mL) or blocking mAb for OX40L or IL-4 (10μg/mL). CD4+ T cells were then stimulated with the MMP-2 peptide pool (2μM) for 6h before intracellular staining of cytokines. Contour plots representing cytokine production by CD4+ T cells are shown for donor CB31 CD4+ T cells primed to MMP-2 alone, or together with rhIL-12, anti-OX40L mAb or anti-IL-4 mAb (not shown). (B) Percentages of MMP-2-specific CD4+ T cells, secreting indicated cytokines, are represented for all donors.

For each cytokine, 5 paired t-tests were used to compare “no conditioning” versus various conditions. p values ≤ 0.01 (*) were considered statistically significant using a Bonferroni correction for 5 comparisons.