Mean %, n, latency and detailed statistic are reported in Table S4.
(A) Examples and densitometric analysis of MCT4, MCT1 and MCT2 quantitative western blots of extracts from dorsal hippocampal punches taken around the needle placement from trained rats that received hippocampal MCT4- or SC4-ODN injections and were euthanized 12 h after training (n=4/group). Data are expressed as mean percentage ± SEM of trained-SC4-ODN (100%) mean values. All MCTs values were normalized to those of actin.
(B) Memory acquisition (acq) and retention are expressed as mean latency ± SEM (in seconds, sec). Hippocampal injection of MCT4-ODN disrupted long-term memory and L-lactate but not glucose, rescued it. MCT4- or SC4-ODN were injected 1 hr before training. L-lactate, glucose or vehicle (PBS) were injected 15 min before training. Rats were tested 24 hr after training (Test 1) and 6 days later (Test 2). (n=10-12/group). * p < 0.05