Table 1.

Variability and efficiency of OPV_12/13 and c-myc real-time PCR assays.

PCR Assay	copy no.	Variability* CT		R2 **	PCR Efficiency (%) **
		intra-assay	inter-assay
OPV 12/13	106	17.58 ± 0.23	17.42 ± 0.20	0.99	96.00
	105	20.33 ± 0.16	20.55 ± 0.20
	104	24.13 ± 0.15	24.34 ± 0.21
	103	27.01 ± 0.08	27.42 ± 0.41
	102	31.22 ± 0.08	31.24 ± 0.38
	101	34.42 ± 0.05	34.96 ± 0.47
c-myc	106	19.63 ± 0.04	19.74 ± 0.46	0.99	97.62
	105	23.23 ± 0.28	23.09 ± 0.31
	104	26.40 ± 0.05	26.61 ± 0.29
	103	29.53 ± 0.02	29.65 ± 0.55
	102	34.73 ± 0.49	33.76 ± 0.97
	101	36.74 ± 0.36	36.39 ± 0.58

* for the determination of the intra-assay variability, plasmid standards (10⁶-10¹ plasmid copies) were measured in triplicate, and for the inter-assay variability, measurements were repeated on 3 consecutive days; ** calculation using the slope of the calibration curves for plasmid standards (10¹-10⁶ plasmid copies); The linear detection range is 10⁶-10¹ copies per run. C_T threshold cycle