Table 4.
Details of oligo nucleotides used in PCR reactions
| Oligo name | Oligonucleotide sequence 5'→3' | S/A | Ta (°C) | Tm (°C) |
|---|---|---|---|---|
| c-myc | 60.0 | |||
| c-myc F | GCCAGAGGAGGAACGAGCT | S | 59.4 | |
| c-myc R | GGGCCTTTTCATTGTTTTCCA | A | 54.2 | |
| c-myc TM | F-TGCCCTGCGTGACCAGATCC-T | S | 65.9 | |
|
OPV12/13/MPXV 82 protein |
62.0 | |||
| OPV F | GCCAATTGTCTTTCTCTTTTACTGA | S | 56.2 | |
| OPV R | GAAAACATTTAAGGATGAATCCATCT | A | 55.4 | |
| OPV TMGB | F-CCTTCTATAGATCTGAGAAT NQF MGB | S | 65.5 | |
| rpo18/polymerase# | 60.0 | |||
| rpo18 F | CTGTAGTTATAAACGTTCCGTGTG | S | 50.7 | |
| rpo 18 R | TTATCATACGCATTACCATTTCGA | A | 47.0 | |
| rpo18 TM | F-ATCGCTAAATGATACAGTACCCGAAXTCTCTACT-PH | |||
| D8L/IMV membrane protein | 60.0 | |||
| D8L F | GGATGTTCTATATACGGGGATGAGTAG | S | 57.9 | |
| D8L R | AAAGTTAATAAGGTAGATGACACGTTCT | A | 58.5 | |
| D8L TM | F-TTCTCATCATCAGAATAAA-NFQ MGB | S | 65.1 |
Y = C or T, B = C or G or T, W = A or T, F = FAM label, T = TAMRA label, TM = hydrolysis probe, TMGB = hydrolysis probe coupled to an MGB moiety; MGB = Minor Groove Binder, NFQ = Non-fluorescent quencher, Tm = Melting temperature (calculated by nearest neighbor method), Ta = Annealing temperature, A = antisense orientation, S = sense orientation, # [7].