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. 2011 Mar 24;11:51. doi: 10.1186/1471-2229-11-51

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Copyright ©2011 Husar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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UGT73C6_pro:GUS expression is present in all organs and is developmentally regulated. (a) A homozygous line expressing a UGT73C6 promoter GUS fusion, that showed a characteristic staining pattern, was chosen for histochemical analysis of UGT73C6_pro:GUS expression in different organs and developmental stages. (b) Response of UGT73C5, UGT73C6, DWF4, ROT3 and BR6ox2 expression in eight-day-old whole seedlings to external application of 1 μM 24-epiBL. GAPC2 was used for standardization. The mean and standard deviation of three biological replicates is shown. UGT73C5 and UGT73C6 expression levels are not statistically significantly altered (t-test p-value > 0.05) in treated versus untreated samples, while the expression of DWF4, ROT3 and BR6ox2 is significantly repressed (t-test p-value < 0.01) by 1 μM of 24-epiBL.