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. 2011 Mar 24;11:51. doi: 10.1186/1471-2229-11-51

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Copyright ©2011 Husar et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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UGT73C6-YFP reporter generation and analysis. (a) Top: Adult phenotypes of independent transgenic lines expressing either a UGT73C6_pro:UGT73C6-YFP construct (center) or a 35S_pro:UGT73C6-YFP construct (right) as compared to wild type (wt), grown for 4 weeks in long-day conditions (16 hrs 80-100 μmol·m^-2·s^-1white light/8 hrs dark) at 21 ± 2°C. Bottom: Western blot analysis of UGT73C6-YFP protein levels in 2-week-old seedlings of the lines whose phenotype is shown above, using an anti-GFP antibody. (b) Representative YFP expression pattern of UGT73C6-YFP analyzed in leaves of eleven-day-old seedlings of line 35S_pro:UGT73C6-YFP/4 and line UGT73C6_pro:UGT73C6-YFP/5 by fluorescence microscopy. The scale bars represent 10 μm.