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Uptake by M. smegmatis of FM5-95 from 7H9 top agar during growth. Cells were imaged at 37°C under 7H9 top agar supplemented with 2 μg/ml FM 5-95. Images were captured using a 560 nm excitation filter every 15 mins over 15 hrs with 50% of the maximum light intensity and the camera sensitivity set to 175. Time of image capture is shown (Scale bars = 10 μm).
