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. 2011 Apr 11;6(4):e18624. doi: 10.1371/journal.pone.0018624

Figure 3. PPy(DBS) supported long term maintenance and differentiation of fetal NSCs.

Figure 3

(A) In the absence of FGF2, fetal NSCs differentiated into astrocytes (GFAP positive); neurons (marked with Tuj1) and oligodendrocytes (labeled for MBP). PPy(DBS) supported NSC differentiation over 7 days in N2 conditions, similar to TCPS. (B) mRNA expression levels analyzed by real-time PCR showed comparable levels of Nestin, GFAP and ß-tubulin relative to TBP for NSCs when cultured on PPy(DBS) and TCPS for 7 days in N2 media supplemented with FGF2, validated by their immunoreactivity for Nestin. (D) Real-time quantitative PCR results showed similar mRNA levels for Nestin, GFAP and ß-tubulin relative to TBP for fetal NSCs grown on TCPS and PPy(DBS). Student's two-tailed t-test was used. The scale bar represents 100 µm.