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. 2011 Apr 11;6(4):e18624. doi: 10.1371/journal.pone.0018624

Figure 4. Electrical reduction of PPy(DBS) caused alterations in polymer surface properties, leading to impaired cell viability.

Figure 4

(A) Fetal NSCs were cultured on the PPy(DBS) films for 2 days before electrical reduction of the substrates at -0.9 V vs. Ag/AgCl. Reduction of PPy(DBS) caused cell death and detachment from the substrate. However, NSC viability on the gold coated areas of the substrates was not impaired. The dashed line indicates the gold/PPy(DBS) border. The scale bar represents 500 µm. (B) NSC viability on substrates reduced prior to cell seeding was lower than on substrates in the pristine state. Reduction of the polymer with live cells led to an even larger decrease in cell viability. The scale bar represents 200 µm. (C) Quantification of the average number of live and dead cells in (B) as calculated from five random 10x images from three separate experiments. 1-Way ANOVA analysis of variance followed by Tukey's multiple comparison test was used.