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. 2011 Mar 16;10:65. doi: 10.1186/1475-2875-10-65

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Copyright ©2011 Limbach et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of P. yoelii proteins from vaccinia-P. yoelii vectors. RK-13 cells were infected with vaccinia vectors that express PY03011 (lane A), PY03424 (lane B), PY03661 (lane C) or PyCSP (lane D), or an "empty" vaccinia vector (lane E). Lysates were run on an acrylamide gel, transferred to a PVDF membrane and probed with antisera from mice immunized with PY03011, PY03424 or PY03661 recombinant proteins. Arrows indicate the major P. yoelii protein products. Molecular weight markers (with kilodaltons designations) are shown in the first lane.