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. 2011 Apr 11;6(4):e18795. doi: 10.1371/journal.pone.0018795

Figure 1. c-Myc plays an early role in appendicular skeletal development.

Figure 1

(A–C') Whole mount in situ hybridization of c-Myc in forelimb buds at E10.5, E11.5 and E12.5 of wildtype and Prx1-Cre c-Myc mutant embryos. (D, D') In situ hybridization for c-Myc in sections of proximal tibia at E13.5 of wildtype and Prx1-Cre c-Myc mutant embryos. (E, E') Higher magnification views of proximal tibia sections shown in D and D'. Cartilage and (C) and perichondrium (P) are indicated. (H, I) BrdU staining in limb buds at E11.5 and proximal tibia at E13.5 respectively. (J) Summary of BrdU-positive cells in the proliferative zone (PZ) and perichondrium (Pc).