Figure 2. Limb bud deletion of c-Myc affects Sox9 and Runx2 expression and formation of cartilage anlage.

Analysis of Sox9 expression (A-D') and Runx2 expression (E-G') by in situ hybridization in forelimbs of Prx1-Cre control and c-Myc cko embryos at the indicated stages. Dashed lines delineate the limb bud. (H, H') Alcian blue staining of forelimb and hindlinb cartilage anlage of control and c-Myc cko embryos at E13.5.