Figure 7. Combined c-Myc and N-Myc deletion mimics deletion of N-Myc alone in the early limb bud and prevents Sox9 upregulation caused by c-Myc deletion.

(IA–D) BrdU labeling of limb buds at E10.5 for the indicated genotypes. Higher magnification views of boxed areas (red) are shown in A'–D'. Similar results were observed in sections from three different limb buds. (IIA–D') pRpCTD immunohistochemistry of E10.5 limb buds of the indicated genotypes with high (IIIA–D) Comparison of limb size and morphology for the indicated strains at E12.5. (IV) Whole mount in situ hybridization for Fgf8, Shh and Sox9 at E10.5 and Runx2 at E11.5 in Prx1-Cre, c-Myc cko, N-Myc cko and dcko embryos.