Table 2. Constructively-active human AKT1 cause convergent extension defects in zebrafish embryos.
Somite width (µm)(n) | Extension angle (°)(n) | |
1× buffer | 173.7±3.3a(78) | 77.7±5.3a(79) |
caakt1 50 pg | 176.4±2.0b(96) | 84.0±4.0b(97) |
caakt1 100 pg | 175.9±2.7b(85) | 82.0±5.8b(89) |
caakt1 150 pg | 202.4±3.0c(75) | 113.0±3.7c(82) |
Embryos were injected without or with different amount of constructively-active human akt1 (caakt1) mRNAs, subjected to WISH against myod and ctsl1b and somite width and extension angle of embryos were measured (N = 4).