Figure 1. Decrease in parasite biomass and IRBC accumulation in organs in infected Rag2^−/− mice.

A) Survival and (B) parasitemia in WT (n = 5) and Rag2^−/− mice (n = 5) infected with PbAluc. Neurologic signs of CM appeared on days 6–12 (shaded area), with death occurring 24–48 h after onset. Parasitemia (%) values are expressed as mean ± SD of 5 mice per group. In vivo bioluminescence imaging quantification of IRBC accumulation in the whole body (C) and head (D) of infected WT and Rag2^−/− mice. Mice were injected with luciferin and IRBC accumulation was measured by bioluminescence imaging as described in Materials and Methods. Each bar represents mean luminescence values (log) ± SD of 5 mice. #p<0.01, log-rank test and **p<0.01 and ***p<0.001, Mann Whitney test. Pseudocolor images of whole body (E) and Head (F) from infected WT and RAG2 KO mice.