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. 2011 Apr 11;6(4):e18720. doi: 10.1371/journal.pone.0018720

Figure 4. Depletion of myeloid cells does not alter IRBC distribution during ECM.

Figure 4

MAFIA mice were infected with PbAluc and injected on days 5, 6, and 7 with the drug AP20187 as described in Material and Methods. (A) Depletion of granulocytes/monocytes (defined as CD45+CD11b+Gr1+) was assessed by flow cytometry on day 7 post-infection. Data plots presented are from one mouse and similar data were obtained for 4 more mice. (B) Survival and (C) parasitemia of treated and non-treated MAFIA mice (5 per group). Neurologic signs of CM appeared on days 6–12 (shaded area), with death occurring 24–48 h after onset. Parasitemia (%) values are expressed as mean ± SD of 5 mice per group. In vivo bioluminescence imaging quantification of IRBC accumulation in the whole body (D) and head (E) of treated and non-treated infected mice. Luminescence values (log) as mean ± SD of 5 mice.