Figure 1. Generation of a TGF-β reporter cell line.

(A) Schematic diagram of pSMPUW-(caga)₁₂-Luc lentivirus based TGF-β reporter vector. (B) Representative luciferase response of 293T-caga cells to recombinant TGF-β. 293T cells were transduced with the pSMPUW-(caga)₁₂-Luc vector, and cultured in the presence of recombinant human TGF-β for 16 hrs. Luciferase activity was measured from the cell lysates. Mean ± S.D. from duplicates are shown. (C) Schematic diagram of generation of 293T-caga-Luc-CD32-CD86 cells and how the cells function as artificial antigen presenting cells.