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. 2011 Apr 11;6(4):e18365. doi: 10.1371/journal.pone.0018365

Figure 4. Investigation of requirement for direct contact between T cells and TGF-β reporter cells.

Figure 4

(A) 293T-caga-Luc-CD32-CD86 cells were mixed with an equal number of un-manipulated 293T cells. Under this condition, a single 293T-caga-Luc-CD32-CD86 cell stimulates CD4 T cells and responds to TGF-β activity produced by the same T cells. (B) 293T-caga cells that did not have CD32 or CD86 were mixed with 293T-CD32-CD86 cells lacking the (caga)12-Luc reporter. Under this condition the 293T-caga reporter cells have minimal contact with CD4 T cells.