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. 2011 Apr 11;6(4):e18365. doi: 10.1371/journal.pone.0018365

Figure 5. TGF-β bioassay from T cell culture supernatants.

Figure 5

(A) Pre-activated CD4+CD25 T cells were re-stimulated with 293T-caga-Luc-CD32-CD86 cells in the presence of anti-CD3 as a co-culture assay. (B) Supernatants were taken from the co-culture assay, added to new wells of 293T-caga-Luc-CD32-CD86 reporter cells and luciferase activity was measured. Error bars represent mean ± S.D. of duplicates.