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. 2011 Apr 11;6(4):e18365. doi: 10.1371/journal.pone.0018365

Figure 8. Effects of anti-LAP or anti-latent TGF-β/pro-TGF-β mAbs on TGF-β activity in T cell culture supernatants.

Figure 8

(A) Recombinant human TGF-β was pre-mixed with anti-active TGF-β Abs (1D11 or chicken anti-TGF-β), an anti-LAP mAb (TW7-20B9), an anti-latent TGF-β/pro-TGF-β mAb (TW7-28G11) (final 50 µg/ml), or ALK5 inhibitor II (final 1 µM), and then added to 293T-caga-Luc-CD32-CD86 reporter cells. (B) A T cell culture supernatant containing an equivalent amount of TGF-β was pre-mixed with the indicated Abs or the ALK5 inhibitor, and added to 293T-caga-Luc-CD32-CD86 cells. The luciferase activity was measured after 16 hr culture. Error bars represent mean ± S.D. of duplicates.