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. Author manuscript; available in PMC: 2012 May 5.
Published in final edited form as: Neuroscience. 2011 Feb 18;181C:117–126. doi: 10.1016/j.neuroscience.2011.02.034

Figure 3.

Figure 3

Location within primary auditory cortex (Au1) of confocal image analyses. (A) Peristimulus time histogram of responses of a neuronal unit (all action potential waveforms depicted in the Inset) to a 34 kHz pure tone (duration of tone marked by dashed vertical green lines). (B) Cresyl violet stain (2X magnification) of lesioned sites (dashed circle) within areas showing primary-like auditory responses (A), presumably corresponding to primary auditory cortex (black box). This site corresponds to −2.54 mm re. Bregma according to the Paxinos and Franklin (2001) mouse brain atlas. (C) DAPI nuclear staining viewed under the confocal microscope at 10X magnification through an anatomically similar slice through primary auditory cortex (white dashed box). (D) Expanded grayscale view of the boxed area in (C) showing nuclear density variations similar to that seen in the cresyl violet stain (B). This enabled targeting of specific, identifiable regions in the middle of the supragranular (layers 1–2), thalamorecipient (layers 3–4) and infragranular (layers 5–6) layers for confocal image analysis (rectangular zones).