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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Org Lett. 2011 Mar 15;13(8):2034–2037. doi: 10.1021/ol200420u

Figure 2.

Figure 2

Summary of anti-filarial activity of 5. (A) In vitro analysis of AsnRS inhibition by 5 against human and filarial AsnRS using pre-transfer editing assay. Lane/Reaction contents: (1) human AsnRS; (2) human AsnRS, 150 μM 5; (3) B. malayi AsnRS; (4) B. malayi AsnRS, 150 μM 5. Assays employed limiting (50%) maximal enzyme activity. (B) Live worms are coiled and motile whereas dead worms (C) are elongated and nonmotile. (D) Live worms reduce MTT which leads to absorption at 510 nm. Lane/Reaction contents: (1) B. malayi, DMSO; (2) B. malayi, 100 μM albendazole as positive control; (3) B. malayi, 100 μM 5.