Table 1.
Modified Rule of Five for FDA-Approved Non-Oral Drugs Generated by the Bootstrap Method
| Administration route |
MW (Da) |
# of H donors | # of H acceptors | log P |
|---|---|---|---|---|
| Ophthalmic | 500 a | 3 | 8 | 4.2 |
| Inhalation | 500 a | 4 | 10 a | 3.4 |
| Transdermal | 335 | 2 | 5 | 5.0 a |
The threshold values for the physicochemical parameters where the Rule of Five statistically proves to be inapplicable were generated by identifying the median values from the bootstrap confidence interval of each of the thresholds below which 90% of the non-oral drugs would fall (N = 10000) (see Supplementary Material).
a
Bootstrap analysis demonstrated that the original Rule of Five values provided appropriate threshold values and did not require changing.