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. Author manuscript; available in PMC: 2012 Apr 29.
Published in final edited form as: J Chromatogr A. 2010 Dec 3;1218(17):2389–2395. doi: 10.1016/j.chroma.2010.11.059

Figure 1.

Figure 1

Separation of proteins from human plasma on DEAE CIM monolithic supports under overloading conditions. CIM DEAE monolithic disk (column volume 340 μL) and tube (column volume 8 mL) were used. Human plasma was four times diluted with Buffer A, and bound proteins were eluted with 0.155 (A) by use of a step gradient. The flow rate was 2 mL/min for the CIM disk, and 10 mL/min for CIM tube. Collected fractions were analyzed by SDS-PAGE. For other separation conditions see Material and methods.