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. Author manuscript; available in PMC: 2012 Apr 29.
Published in final edited form as: J Chromatogr A. 2010 Dec 3;1218(17):2389–2395. doi: 10.1016/j.chroma.2010.11.059

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

Separation of proteins from human plasma on DEAE weak anion-exchanger CIM monolithic supports under overloading conditions. CIM DEAE monolithic disk (column volume 340 μL) was used. Human plasma was four times diluted with Buffer A, and bound proteins were eluted with 0.155 (A), 0.2 (B), 0.3 (C) and 1 M NaCl (D)* by use of a step gradient. The flow rate was 2 mL/min. Collected fractions were analyzed by SDS-PAGE. For other separation conditions see Material and methods.

*After injection of 37 μL plasma (6.5 mg protein/mL support), the amount of material eluted with 1M NaCl was not sufficient for subsequent SDS-PAGE