Skip to main content
. Author manuscript; available in PMC: 2012 Apr 29.
Published in final edited form as: J Chromatogr A. 2010 Dec 3;1218(17):2389–2395. doi: 10.1016/j.chroma.2010.11.059

Figure 3.

Figure 3

Two dimensional electrophoretic separation of fractions eluted with 0.155 M NaCl from CIM DEAE disk-shaped monolithic column (column volume 340 μL) and displacement of HSA under overloading conditions.

A–0.25 mL plasma containing about 15 mg protein was loaded on the column (for comparison, see lane No. 1 in Figure 1A);

B–1.06 mL plasma containing 187.5 mg protein was loaded on the column (for comparison, see lane No. 8 in Figure 1A).

For separation conditions, see Figure 1 and Material and methods.