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. Author manuscript; available in PMC: 2012 Apr 29.
Published in final edited form as: J Chromatogr A. 2010 Dec 3;1218(17):2389–2395. doi: 10.1016/j.chroma.2010.11.059

Figure 5.

Figure 5

Figure 5

Figure 5

Figure 5

Separation of proteins from human plasma SO3 strong cation-exchanger CIM monolithic supports under overloading conditions. CIM SO3 monolithic disk (column volume 340 μL) was used. Human plasma was four times diluted with Buffer A, and bound proteins were eluted with 0.2 (A), 0.3(B), 0.5(C) and 1 M (D) NaCl by use of a step gradient. The flow rate was 2 mL/min. Collected fractions were analyzed by SDS-PAGE. For other separation conditions see Material and methods.