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. Author manuscript; available in PMC: 2012 Apr 29.

Published in final edited form as: J Chromatogr A. 2010 Dec 3;1218(17):2389–2395. doi: 10.1016/j.chroma.2010.11.059

Use of 100 μL monolithic SO₃ strong cation-exchanger CIM disk for separation of proteins from human plasma under overloading conditions. Human plasma was four times diluted with Buffer A, and bound proteins were eluted with 0.2 (A), 0.3(B), 0.5(C) and 1 M (D) NaCl by use of a step gradient. The flow rate was 1 mL/min. Collected fractions were analyzed by SDS-PAGE. For other separation conditions see Material and methods. Band labeled in the Figures were excised, digested by trypsin and used for identification by LC-MS/MS. Some identified proteins are listed in Table 1.

Use of 100 μL monolithic SO₃ strong cation-exchanger CIM disk for separation of proteins from human plasma under overloading conditions. Human plasma was four times diluted with Buffer A, and bound proteins were eluted with 0.2 (A), 0.3(B), 0.5(C) and 1 M (D) NaCl by use of a step gradient. The flow rate was 1 mL/min. Collected fractions were analyzed by SDS-PAGE. For other separation conditions see Material and methods. Band labeled in the Figures were excised, digested by trypsin and used for identification by LC-MS/MS. Some identified proteins are listed in Table 1.