Fig. 3.
Effect of RR110 on the in vitro translation efficiency of mRNAs comprising a G-quadruplex motif in their 5′ UTR. (A) Chemical structure of RR110. (B) Relative in vitro translation efficiencies of NRAS UTR(−)Q, NRAS UTR(+)Q and NRAS UTR(+120)Q mRNAs in the presence of the indicated amounts of RR110, as measured by luciferase activities. For each construct, the measured luciferase activity was normalized to the activity obtained in the absence of the ligand, which was set as 100%. Experiments were performed at least three times using at least two separate batches of RNA. The average values are presented along with the standard error on the mean.