Table 2.
Transfer Efficiency of F ΔTraI plasmid complemented with TraI
| Donor and recipient co-incubation time |
||
|---|---|---|
| TraI variant | 30 min | 60 min |
| TraI wt | 0.039 ± 0.007 | 0.12 ± 0.04 |
| TraI Y16F | ND | 2.5 ± 1.6 × 10−5 |
| TraI Y17F | 8.6 ± 2.0 × 10−3 | 0.037 ± 0.004 |
| TraI Y23F | 0.033 ± 0.032 | 0.063 ± 0.050 |
| TraI Y24F | 0.028 ± 0.011 | 0.062 ± 0.009 |
| TraI Y16F/Y17F | <1.7 × 10−5 | 1.5 × 10−5 |
| TraI Y23F/Y24F | 0.016 ± 0.001 | 0.033 ± 0.001 |
| TraI Y16F/Y23F/Y24F | ND | 0.5 ± 0.3 × 10−5 |
| TraI Y17F/Y23F/Y24F | ND | 0.07 ± 0.05 |
| TraI Y16F/Y17F/Y23F/Y24F | ND | 1.3 ± 1.4 × 10−5 |
| TraI from ER2738 | 0.090 ± 0.012 | 0.29 ± 0.07 |
| No TraI | ND | <1.6 × 10−7 |
Transfer efficiencies, calculated as number of transconjugants per donor, are averages of 2–4 independent measurements and are listed with the standard deviation. The E. coli K12 ER2738 strain was used as a control for wt F plasmid transfer efficiency. ND, not determined.