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. 2010 Dec 3;39(7):2559–2570. doi: 10.1093/nar/gkq1174

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — AFM imaging of conventional (H2A) and chimeric H2A.ddBbd nucleosomes. A 255-bp 601 DNA sequence was used to reconstitute centrally positioned conventional and H2AddBbd nucleosomes. The nucleosome samples were then visualized by AFM. Representative AFM images for the conventional (nuc H2A) and chimeric (nuc H2AddBbd) nucleosomes are shown in (A) and (B), respectively. (C) The complexed DNA length (L_c) distribution for conventional and H2AddBbd nucleosomes is presented. Note that Lc peaks at ∼130 and ∼150 bp for the H2A.ddBbd and conventional nucleosomes, respectively. (D) Nucleosome position (DL) distribution for conventional and H2AddBbd nucleosomes. The numbers of particles used for the calculation of the distributions were N = 3247 and 971 for conventional and H2A.ddBbd nucleosomes, respectively.