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. 2010 Nov 24;39(7):2503–2518. doi: 10.1093/nar/gkq1178

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Catalytic activity of MK2 is not necessary for rescue of p38α-MSK1 signaling but indispensable for rescue of the full IEG response. Immortalized MK2/3 DKO MEFs stably transduced with MK2, MK2-K79R or GFP alone were starved over night and then were left non-stimulated or were stimulated with anisomycin (10 µg/ml) or UV (200 J/m²). (A) Rescue of anisomycin-stimulated p38/MSK1/CREB signaling by both, MK2 and catalytic inactive MK2-K79R in MK2/3 DKO MEFs after 30 min of treatment. Phosphorylation of Hsp25 cannot be rescued by MK2-K79R. (B) Rescue of anisomycin- and UV-induced IEG expression (egr1, c-fos, TTP) in MK2/3 DKO MEFs by MK2 and MK2-K79R. Transcript levels after 60 and 90 min were determined by RT-PCR, normalized to the actin signal and displayed. Representative data from six independent experiments performed in duplicates are shown. The inhibitory concentration of anisomycin leads to a more sustained IEG expression while UV-treatment and subinhibitory anisomycin concentration (10–50 ng/ml, not shown) yield a more transient IEG response (cf. Supplementary Figure S3).

Figure 4. — Catalytic activity of MK2 is not necessary for rescue of p38α-MSK1 signaling but indispensable for rescue of the full IEG response. Immortalized MK2/3 DKO MEFs stably transduced with MK2, MK2-K79R or GFP alone were starved over night and then were left non-stimulated or were stimulated with anisomycin (10 µg/ml) or UV (200 J/m²). (A) Rescue of anisomycin-stimulated p38/MSK1/CREB signaling by both, MK2 and catalytic inactive MK2-K79R in MK2/3 DKO MEFs after 30 min of treatment. Phosphorylation of Hsp25 cannot be rescued by MK2-K79R. (B) Rescue of anisomycin- and UV-induced IEG expression (egr1, c-fos, TTP) in MK2/3 DKO MEFs by MK2 and MK2-K79R. Transcript levels after 60 and 90 min were determined by RT-PCR, normalized to the actin signal and displayed. Representative data from six independent experiments performed in duplicates are shown. The inhibitory concentration of anisomycin leads to a more sustained IEG expression while UV-treatment and subinhibitory anisomycin concentration (10–50 ng/ml, not shown) yield a more transient IEG response (cf. Supplementary Figure S3).