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. 2010 Nov 24;39(7):2503–2518. doi: 10.1093/nar/gkq1178

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Egr1 promoter-reporter assays in MK2/3- (A and B) and MK2-deficient (C) MEFs. (A) Egr1-370-Luc (cf. Figure 7C) reporter activity in MK2/3 DKO MEFs rescued with MK2 (white columns) or GFP (black columns) 4 h after stimulation with anisomycin (25 ng/ml) or PMA. (B) Anisomycin-stimulated Egr1-370-Luc reporter (black columns) and Egr1-370-SRFmut-Luc (white columns) activity in MK2/3 DKO MEFs rescued with MK2, MK2-K79RF or GFP. (C) Stably SRE.l-transfected MK2-deficient (white bars) and WT control (black bars) MEFs were serum starved over night and were left unstimulated or were stimulated with anisomycin (aniso) for 5 h. For inhibition of p38, 5 µM SB202190 (SB) was added 1 h prior to stimulation. Reporter luciferase activity was determined, normalized to total protein and induction was calculated. Representative data from three independent experiments are shown.