Table 1.
List of the 27 different anisomycin-induced genes with >2-fold increased MK2-dependent transcript levels in MEFs
| Gene description | Fold induction |
Ratio MK2/ GFP | Position of promoter elements |
||
|---|---|---|---|---|---|
| GFP | MK2 | SRE | CRE | ||
| Zinc finger protein 36 (TTP) | 2.70 | 33.66 | 12.49 | −24 | −2203 |
| Early growth response 2 | 31.9 | 383.66 | 12.04 | −75, −126 | −62 |
| Early growth response 3 | 8.91 | 102.95 | 11.56 | −465, −799 | −583 −634 −2460 −2594 |
| Calponin 3, acidic | 0.37 | 3.49 | 9.32 | −68, −86, −134, −488, −613 | − |
| ADP-ribosylation factor 4 | 0.69 | 5.98 | 8.66 | −285 | −2, −64 |
| Kinesin family member 5B | 0.58 | 3.58 | 6.11 | −562, +33 | − |
| Jun-B oncogene | 3.01 | 16.26 | 5.41 | −61, −318 | −105 |
| DnaJB1 | 0.78 | 3.43 | 4.37 | −82, −244, −704, −796, −826, +1 | − |
| Cell division cycle associated 4 | 0.80 | 3.47 | 4.36 | −10, −485 | − |
| Forkhead box O3 | 1.39 | 5.83 | 4.21 | −414 | −16, −955, −966, −1090 |
| Methionine adenosyltransferase II, α | 0.84 | 3.46 | 4.10 | −103, −861 | − |
| Suppressor of cytokine signaling 3 | 1.14 | 3.94 | 3.45 | −737 | − |
| Nuclear pore membrane protein 121 | 1.51 | 4.91 | 3.26 | −565, −576 | −360, −378 |
| Sirtuin 1 | 1.82 | 5.03 | 2.77 | −495, −721 | − |
| PMA- induced protein 1 | 2.97 | 8.09 | 2.72 | −397 | −59, −66 |
| FBJ osteosarcoma oncogene (c-fos) | 43.7 | 116.2 | 2.66 | −295 | 75, −175, −301, −349 |
| Sprouty homolog 2 (Drosophila) | 2.43 | 6.33 | 2.61 | −249, −460, −626, −769 | −593 |
| Transducer of ErbB-2.1 | 1.45 | 3.53 | 2.44 | −930 | −2280 |
| Insulin induced gene 1 | 1.46 | 3.25 | 2.22 | −122 | −193 |
| Immediate early response 5-like | 3.27 | 7.25 | 2.22 | −63, −127, −985 | − |
| Transformed mouse 3T3 cell double minute 2 | 1.54 | 3.41 | 2.21 | −806 | − |
| Metallothionein 1 | 2.27 | 5.00 | 2.20 | −870, −961 | − |
| Sorting nexin 5 | 1.50 | 3.24 | 2.17 | − | 327, −367 |
| Zinc finger and BTB domain containing 11 | 1.88 | 4.08 | 2.17 | −48, −60, −266, −618, −775, −913, −953 | − |
| Vascular endothelial growth factor A | 2.82 | 5.81 | 2.06 | −230, −265, −303, −694 | −445 |
| Cyclin-dependent kinase inhibitor 1A | 1.67 | 3.42 | 2.05 | −568, −790, +45 | −274 |
| Immediate early response 2 | 16.9 | 34.54 | 2.05 | −29, −511, −534, −725 | −48, −1211 |
Genes identified in gene array were filtered and sorted as described in the text. The fold of induction of the transcript level before and after 90 min of stimulation by 10 µg/ml anisomycin is given for MK2/3 DKO MEFs rescued with MK2 or GFP alone. The ratio between transcript levels of stimulation of MK2 and GFP rescued cells is calculated and used for ranking. SRE and CRE promoter elements identified are given. The numbers represent the relative position of the central nucleotide of the element to the major transcriptional start site (‘−’—no promoter element identified).