Figure 2.

Cyclin E is not immobilized on the nuclear matrix in cancer cells. (A) Western blot showing absence of cyclin E from the DNase resistant fraction in representative cancer cell lines fractionated by the procedure used in Figure 1. (B) Immunofluorescence images of RT4 cells showing cyclin E (green) and DNA (blue). Cyclin E occupies a focal pattern in mock treated cells, but is fully extracted by DNase. Bar is 5 μm.