Figure 5.

Idr1 and Idr2 bind at neighboring sites in the genome to influence transcription. (A) The heatmap shows the gene expression profiles of genes bound by both Idr1 and Idr2. Data shown are for genes differentially expressed in the Δidr1Δidr2 double mutant, Δura3 parent strain, and Δidr1 and Δidr2 single mutant strains. GO terms and gene text colors correspond to functional categories as shown in the legend (similar to Figure 4). Asterisks represent those genes with putative cis-regulatory motifs within 500 bp. The red numbers to the left of the hierarchal clustering data in (A) refer to clusters described in the text. The diagrams to the left of the clustering heatmap suggest putative network topologies for how Idr1 and Idr2 could work together (see also text descriptions). Dotted arrow indicates an indirect influence of Idr2 over idr1 gene expression as shown in Supplementary Figure 4. Pointed arrows indicate activation influences, whereas blunted arrows indicate repression influences. (B) Circle chart indicates the overlap between the three genome-wide data sets.