Table 1.

Binding of E2 and E2ΔLL to specific and non-specific DNA

E2 site A:T spacer	41 ± 13	22 ± 7
Non-specific DNA	1203 ± 437	110 ± 25
Specificityb	29.4	5.0

^aThe apparent equilibrium constant (K_eq(apparent)) was obtained using the equation:

[bound_DNA] = [maximum bound_DNA] [protein]/([protein] + K_eq(app))

When [DNA] is much less than the K_eq, the apparent equilibrium constant is equal to the protein concentration at half maximum DNA binding. The values shown are derived from five independent experiments.

^bThe specificity is the ratio of non-specific to specific binding (42):

Specificity = K_eq(app)^{non-specific DNA}/K_eq(app)^{specific A:T spacer}.