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. 2010 Dec 3;39(7):2969–2980. doi: 10.1093/nar/gkq1217

Table 2.

Kinetic data for E2- and E2ΔLL–DNA complex formation obtained using stopped-flow fluorescence experiments

Fast rate Slow rate
k1 on (M−1 s−1) k1 off (s−1) k2 (s−1)
HPV 6 E2 (9.2 ± 0.8) × 107 68 ± 16 29 ± 6
HPV 6 E2ΔLL (1.0 ± 0.1) × 109 182 ± 33 44 ± 18

The k1 on and k1 off of the fast rate were obtained from the slope and intercept with the y-axis of the graph shown in Figure 6. The k2 value was obtained from the slope of the slow rate on the same graph. The values for HPV 6 E2 are from previously published data (13).