Figure 1.

Sld2T84D binds to ssARS1. (A) Schematic of ARS1. The complete nucleotide sequences are found in Supplementary Table S1. (B) Purified GST–Sld2T84D, GST–Sld2 or GST were studied for interaction with radiolabeled DNA as indicated to the left of the gel. GST–Sld2T84D, GST–Sld2 or GST (13 pmol) and varying amounts of DNA, as indicated in the figure, were mixed with 1.3 mg glutathione agarose beads and incubated for 5 min at 30°C. The beads were washed and analyzed as described in ‘Materials and Methods’ section. The results from experiments similar to (B) were quantified and plotted as pmol of DNA bound versus pmol of input DNA (C, D and E). Sld2T84D binds tighter to ssARS1-1 than to either ssARS1-2 or dsARS1. Sld2T84D binds tighter than Sld2 to ssARS1-1.