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. 2010 Nov 24;39(7):2580–2592. doi: 10.1093/nar/gkq1222

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Sld2T84D binds to ssARS305 bottom strand (ssARS305-1). (A) Purified GST–Sld2T84D, GST–Sld2 or GST were studied for interaction with radiolabeled ssARS305-1 (top panel), ssARS305-2 (middle panel) or dsARS305 (bottom panel). GST–Sld2T84D, GST–Sld2 or GST (13 pmol) and varying amounts of DNA, as indicated in the figure, were mixed with 1.3 mg glutathione agarose beads and incubated for 5 min at 30°C. The beads were washed and analyzed as described in ‘Materials and Methods’ section. The results from experiments similar to (A) were quantified and plotted as pmol of DNA bound versus pmol of input DNA (B and C). Sld2T84D binds tighter to ssARS305-1 than to either ssARS305-2 or dsARS305. Sld2T84D binds tighter than Sld2 to ssARS305-1.