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. 2010 Dec 6;39(7):2880–2889. doi: 10.1093/nar/gkq1240

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Spreading of miRNA-triggered silencing from phloem companion cells. (A) SUC2:amiR-SUL and SUC2:siR-SUL plants present similar bleaching patterns. (B) UV-induced red chlorophyll autofluorescence is suppressed in bleached areas, which appear light green in a SUC2:amiR-SUL leaf. Arrows point to leaf veins. (C) SUC2:amiR-SUL SUC2:3xYFP leaf. Top, visible light; bottom, UV fluorescence. Bright green YFP signal is more restricted than the bleached areas that are dark. (D) Comparison of mild and severely bleached plants. A single leaf is shown in detail. (E) sRNA blots probed with an oligonucleotide specific for amiR-SUL (SUL) or a CH42 fragment (CH42 frag). U6 was used as loading control.