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. 2010 Dec 7;39(7):2571–2579. doi: 10.1093/nar/gkq1254

Figure 2.

Figure 2.

AFM visualization of dinucleosome sliding by RSC. AFM images for dinucleosomes incubated for 60 min at 29°C with the remodeling complex RSC and (a) in the absence of ATP and (b) in the presence of ATP. Typical AFM visualizations of (c) positioned dinucleosomes in the absence of ATP and (d) the five dinucleosome states observed in the presence of RSC and ATP (see text). (e) Schematic of the various dinucleosome states identified on the AFM images. The dinucleosomes have been reconstituted on two 601 positioning sequences separated by 58 bp DNA linker and with 63 bp on one side and 81 bp on the other side. States #1–#5 correspond to the five states illustrated on the AFM images of (d).