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. 2010 Dec 7;39(7):2571–2579. doi: 10.1093/nar/gkq1254

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RSC sliding of trinucleosomes. (a) AFM images of trinucleosomes before and after the incubation with RSC and ATP. (b) Schematic of the trinucleosome construction used. The DNA template contains three 601 positioning sequences separated by 50 bp DNA linker and with 25 bp on each side. The total length is 591 bp of DNA. Schematic of two main trinucleosome configurations identified on the AFM images for a strong sliding condition (high RSC concentration and large reaction time) corresponding to the stationary state. The observed proportion of trinucleosomes in each final State (#A and #B) is reported for a total number of N = 174 trinucleosomes counted.