Figure 1.

ParB contains a C-terminal DNA-binding domain. (A) SDS–PAGE of limited digestion products of ParB. Full-length ParB protein (24 μg) in the absence and presence of 10 μg R4–18 DNA was digested by 0, 0.004, 0.016 and 0.064 μg trypsin for 24 h at 20°C. The SDS–PAGE was stained with Coomassie blue. The structure core resistant to digestion covers residues ∼67–129. (B) Native gel analysis of DNA-protected ParB digestions. The gel was stained by ethidium bromide. Free DNA and DNA complexes with full-length ParB and its structural core are indicated.